TLC silica gel plate should understand its characteristics and advantages and disadvantages before use, and then understand what problems should be paid attention to when using it.

Thin-layer silica gel plate has now been widely used in medicine, pesticide, Chinese herbal medicine and other industries, and is also widely used in new materials and other fields, and plays an important role in the evaluation of gutter oil. The thin silica gel sheet is extruded by the extrusion molding machine, and the extruded silica gel sheet can be cut freely.

Thin-layer silica gel plate has high mobility, low cost, high yield and uniform thickness. The size of the silica gel plate is fixed by the silica gel mold, which is vulcanized at high temperature. The area of the silica gel plate is limited, and the length and width are fixed.

The following aspects should be paid attention to when using the silica gel plate:

1. Because the thin layer chromatography silica gel plate is dry plate, the hand should be smooth when laying the plate, otherwise it is easy to be uneven. When putting the board, it should be smooth, otherwise it will walk out of the oblique line and affect the next work.

2. Point sample capillary can not be used in cross, in the point sample of several points in a straight line, the size must be appropriate.

3. The general solution will be relatively thin, a point sample if not enough also need to repeat the point sample, and should wait until the solvent of the previous point sample volatilized, to avoid sample point is too large, affecting the separation effect.

The above is the problem that the thin layer chromatography silica gel plate needs to pay attention to when using it. As long as these aspects are done well, it will not affect the use of thin layer chromatography silica gel plate in the next.